Department of Microbiology, Government Medical College, Ananathapuram, India
Research Article
Comparison of NS - 1 Antigen Detection by RDT and ELISA and its Correlation with RT PCR for Early Diagnosis of Dengue and Detection of Concurrent Serotypes DENV - 1, 2, 3 in Ananthapuramu District
Author(s): Adireddi Paradesi Naidu*, Chitralekha Saikumar, G Swarnalatha, Sardar Sulthana and Sailaja Meeniga
Introduction
Early diagnosis of dengue is important for appropriate clinical management and vector control. Different serological tests based on the principle of immunochromatography and Enzyme - Linked Immune Sorbent Assays (ELISA) is commonly used for detection of antigen and antibodies of dengue virus. The performance of these tests depends on the sensitivity and specificity. Hence, the study was undertaken to compare nonstructural protein - 1 (NS1) antigen detection by rapid and ELISA correlation with Real Time - Polymerase Chain Reaction (RT - PCR) for diagnosis of dengue and to detect serotypes by RTPCR Serotyping (1 - 4) kit at Tertiary care hospital, Ananthapuramu.
Materials and Methods
In this study 100 clinical suspected cases of Dengue were enrolled. All the collected sera a sample were subjected for NS1 antigen dete.. View More»
