To Evaluate the Drug Resistance Pattern in various Antibiotics with Biofilm-Producing Capability of Klebsiella Pneumonia


Kushali R, Vadivel Jayanth

Aim: Objective of study was to evaluate the drug resistance pattern in various antibiotics with the biofilm-producing capability of Klebsiella pneumoniae. Methods: Klebsiella pneumoniae was isolated for identification from total of 83 OPD and IPD patients by analysing colony morphology, microscopic examination, and performed biochemical testing. Antibiotics susceptibility Tests and biofilm-producing capacity was done by the Kirby-Bauer disk diffusion method and adherence quantitative assays. Chi-square test was used and P < 0.05 was measured as statistically significant.

Results: Out of 83 isolates, 47 (56.6 %) were Biofilm Producer and 36 (43.4 %) were non-biofilm producers by tissue culture method, and tube method 62.7 % and 37.3 % respectively. Among biofilm producer and non-biofilm producer isolates, Klebsiella pneumoniae had the most Resistant to Ceftazidime (100 %) and (61.0 %), followed by Ceftriaxone (56.0 %) and (95.7 %) respectively. The association between biofilm and antibiotic resistance was checked to be statistically significant for aminoglycosides, fluoroquinolones, Cephalosporin’s, Imipenem, Meropenem, Amoxyclav, and Piperacillin Tazobactam. No resistance was observed against Colistin and Polymyxin B.

Conclusion: The strain Klebsiella pneumoniae isolated from different samples showed drug resistance to all broad-spectrum antibiotics whereas there is no drug resistance was found with narrow - spectrum drugs. This study supports the use of narrow-spectrum antibiotics for most of infections.