Author(s): Inder Raj Itagi1 , Ramesh B. H 2 , Ambika H. K3

Dengue Fever is caused by Dengue Viruses (4 Serotypes) by the bites of aedes aegypti mosquito. Laboratory findings in dengue cases show leucopenia and thrombocytopenia which is mild in nature. In this study we have made an attempt to compare platelet count in seropositive and seronegative dengue cases in and around Raichur District. AIMS AND OBJECTIVES: To compare the platelet count in seropositive and seronegative dengue fever patients. MATERIALS AND METHODS USED: Automated cell counter (SYSMEX-5PART). Specimen: Blood or serum in a red top tube. Acute and convalescent specimens do not need be sent together. Collection: KHEL Serology kit with the yellow (red) top blood tubes or any other red topped, clot separator blood tubes. Volume: 2 cc (ml.) of centrifuged serum or plasma. Storage: On ice or in refrigerator (not in a freezer) until it is delivered to CDC Dengue Branch. Any specimens stored greater than a month prior to arrival at CDC will not be tested. Timing of collection for serology: Acute- obtained up to 5 days after onset of symptoms; convalescent- 6 or more days after the onset of symptoms. Test results are normally available 3 days (PCR) to 1 week (serology) after specimen receipt. During periods of a severe dengue epidemic it may be necessary to prioritize testing based on the severity of disease. Any severe case that is hospitalized should be indicated on the form. Type of Study: PROSPECTIVE (COHORT STUDY) Duration of Study: 6 months (Dec-2011 to May 2012). Study Site: RAICHUR DISTRICT IN KARNATAKA.

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