Author(s): Rabia Parveen Siddiqui1, Minal Wasnik2, Vanita Bhaskar3, P. K. Patra4, Swati Hiwale5
Immunophenotyping of leukemia by flowcytometry offers a better classification of the hematopoietic lineage of malignant cells as compared to morphology. AIM: To determine the immunophenotypic subtypes of acute leukemia in a tertiary care teaching hospital. MATERIAL & METHODS: A one year study of morphologic & flowcytometric data of patients with acute leukemia. RESULTS: Total numbers of acute leukemia patients diagnosed morphologically were 45, out of which 20 patients underwent immunophenotyping by flowcytometry. Maximum patients were in the age group of 0-10 yrs followed by 11-20 yrs with males outnumbering female. Immunophenotypically they belonged to ALL-B cell lineage, ALL-T cell lineage, AML, biphenotypic, inconclusive. CONCLUSION: Immunophenotyping of acute leukemias by flowcytometry, not only helps to confirm the morphologic diagnosis but also helps in assigning specific lineage to the blasts, particularly in acute lymphoid leukemia.
