IDENTIFICATION OF NONFERMENTATIVE GRAMÔ??NEGATIVE BACILLI ISOLATED FROM CLINICAL SPECIMENS

Abstract

Chithra Jayaprakash1, Nadiya Ummer2

BACKGROUND
Nonfermentative Gram-negative bacilli (NFGNB), once regarded as contaminants, have now gained high relevance as opportunistic pathogens which are usually multidrug resistant.
AIM
Identification of nonfermentative Gram-negative bacilli isolated from clinical samples during the study period and to determine the antibiotic susceptibility pattern of Pseudomonas aeruginosa.
MATERIALS AND METHODS
The study was conducted in the Department of Microbiology at a Tertiary Care Teaching Hospital for a period of 6 months. NFGNB isolated from clinical samples were identified by standard procedures and antibiotic susceptibility test was performed for the most frequently isolated nonfermenter, P. aeruginosa.
RESULTS
Majority of the NFGNB isolates were recovered from pus samples (36.36%), miscellaneous specimens ranking second (33.52%). P. aeruginosa was the most common isolate (76.70%) followed by Acinetobacter baumannii (15.90%). P. aeruginosa isolates were most susceptible to piperacillin/tazobactam (71.85%), followed by meropenem (71.11%), and least susceptible to tobramycin (31.85%).
CONCLUSION
As NFGNB have emerged as an important group of organisms responsible for causing multidrug resistant infections particularly in hospitals, their identification to the species level is necessary. Maintenance of a high quality of infection control practices and judicious use of antibiotics are the cornerstone in the control of these microbes.

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