Arup Bhaumik1, Shikha Das2, Sandeep Roy Sarkar3, Payel Chakraborty4, Biswajit Chowdhury5
ABSTRACT: The aim of this study was to diagnose acute leukemia in the population of Tripura using 3-lasers 7-color Flowcytometer. Immunophenotyping was done on peripheral blood samples of acute leukemia cases, by using 2-tube basic AML antibody panel. Samples collected from Regional Cancer Centre, Agartala included in this study 15 Acute Leukemia cases, reported during 2013 & 2014. Among them, 4 were ALL and 11 were AML cases. Out of 15 cases, 12 were adults (9 men and 3 women ranging from 17 to 77 years of age) and 3 were from pediatric patients. A strong correlation (r=0.92) is obtained between the blast cell count by microscopy &flowcytometry. Moreover, this Immunophenotyping procedure on peripheral blood samples also allowed an efficient discrimination between the various cell lineages and facilitated the accurate leukemic blast cell percentage.