Author(s): Venuturumilli Lakshmi Kameswari1, Venuturumilli Ravi Shankar2, K. Narahari Rao3, Souris Kondaveti4
Inflammation is not a disease but a non-specific response of the body defence1. Anti-inflammatory drugs have become popular because of their ability in controlling the inflammatory reaction and mitigating the suffering in such clinical situations. Edema represents the early phase of inflammation.2 In the present study acute and subacute experimental methods were compared with standard drug Indomethacin. Rat hind paw edema, formalin induced peritonitis in mice were used as acute methods, paper disc induced granuloma in rats was used as subacute method. Institutional animal ethics committee permission was taken for all the methods as per CPCSEA guide lines. Inflammation was induced by the following 3 methods: i) Hind paw edema was produced by sub plantar injection of 0.1ml of1% carrageenin3 and paw volume was measured by digital plethysmometer at 0, 3hours. ii) Intraperitoneal injection of 1.5% formalin3 was given to albino mice in peritonitis method and ascitic fluid was measured after 6hours by sacrificing the animals. iii) Under ether anesthesia, sterilized &weighed paper discs were implanted subcutaneously in each axilla and groin of male wistar rats sutured under sterilized conditions in granuloma4 method. The discs were cleared of extraneous tissue, dried and weighed on the 5th day by sacrificing the animals. In all the methods 6 animals in each group (test, standard and control) were taken. Results were tabulated in each method separately and statistical analysis was done by student t test. P value < 0.05 considered significant. Percentage inhibition in each method was calculated.