Ruby Naz 1 , Mohammad Khalid Farooqui 2 , Ruchi Girotra 3 , Mayank Yadav 4 , A. K. Malik 5 , Ashok Kumar 6

INTRODUCTION The purpose of the present study was to determine the microbiological profile and antimicrobial susceptibility pattern of isolates from discharge in CSOM. MATERIAL AND METHODS This study included a total of 187 patients of CSOM with unilateral or bilateral discharge attending department of ENT in SHKM Govt. Medical College and Hospital Nalhar, Mewat, Haryana March 2015 to August 2015. Samples were inoculated on blood and Mac Conkey agar for 24- 48hrs and identification of organism was done by using standard biochemical reactions and antibiotic susceptibility testing done by using modified Kirby Bauer method as per CLSI guidelines. RESULT Among 187 patients included in the study, most of the patients were between age group 11-20 years. CSOM was found to be more common in female patients (52%) than in male (48%) patients. The most common organisms were: Staphylococcus aureus (36%); Pseudomonas aeruginosa (34%); Klebsiella species, (6%) Proteus species (5%); Escherichia coli (4%); and other bacteria like Coagulase negative Staphylococcal species; Citrobacter spp, Enterobacter spp,Enterococcus spp,. According to antimicrobial susceptibility testing, Staphylococcus aureus was more sensitive to ciprofloxacin and gentamycin. Majority of gram negative isolates were sensitive to imipenem, quinolones and amikacin. CONCLUSION Result of our study showed high prevalence and resistance rate of Staphylococci and Pseudomonas isolates from CSOM patients to ß- lactam and other commonly used antimicrobials. Our study suggested that Amikacin, Piperacillin-Tazobactam and quinolones are best choice in these cases. Therefore an appropriate knowledge of antibacterial susceptibility of microorganism may contribute to rational antibiotic use and the success of treatment for CSOM