Benudhar Pande1, Sumitra Mansingh2, Soumyashree Padhan3
Background: Worldwide infertility affects 8 % - 10 % of couples. In recent years, assessment of ovarian reserve has become one of the important tests for diagnosis of infertility. Biochemical tests for follicle stimulating hormone (FSH), anti-mullerian hormone (AMH), luteinizing hormone (LH) and estradiol (E2) are important in assessment of ovarian reserve.
Methods: This is a hospital based prospective observational study conducted in the Department of Obstetrics and Gynaecology, VIMSAR, Burla from November 2018 to October 2020. The study was done on 70 infertile women with regular menstrual cycle. AMH estimation was done using the Beckman Coulter Access® 2 Automated Immunoassay System. Serum FSH and LH were estimated by ADVIA Centaur CP auto analyzer. Correlation between serum AMH, FSH, LH, LH / FSH ratio with body mass index (BMI) and different age groups were performed with appropriate statistical tests.
Results: In our study, we observed that serum AMH levels and LH/FSH ratio were significantly decreased in patients with advanced age, but basal FSH and LH levels were significantly increased according to age. Serum AMH and serum FSH showed a significantly negative correlation between each other. Serum AMH and LH / FSH showed a positive correlation but not statistically significant. Serum FSH and LH/FSH showed a significantly negative correlation.
Conclusions: Serum AMH levels and the LH / FSH ratio were decreased with increase in age. Serum FSH and LH levels were significantly increased according to age. Serum AMH is a better predictor of ovarian reserve as it is relatively stable throughout the cycle and can be assayed at different times during the cycle.